Coding

Part:BBa_K5374013:Design

Designed by: Langning Xu   Group: iGEM24_TestDaily-I-Beijing   (2024-09-16)


DDR-EGFP (Discoidin Domain Receptor). A receptor tyrosine kinase activated by collagen, fused with E


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 253
    Illegal EcoRI site found at 337
    Illegal EcoRI site found at 820
    Illegal PstI site found at 259
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 253
    Illegal EcoRI site found at 337
    Illegal EcoRI site found at 820
    Illegal NheI site found at 835
    Illegal PstI site found at 259
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 253
    Illegal EcoRI site found at 337
    Illegal EcoRI site found at 820
    Illegal BglII site found at 459
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 253
    Illegal EcoRI site found at 337
    Illegal EcoRI site found at 820
    Illegal PstI site found at 259
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 253
    Illegal EcoRI site found at 337
    Illegal EcoRI site found at 820
    Illegal PstI site found at 259
    Illegal NgoMIV site found at 588
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1478


Design Notes

Sequence was designed for stable EGFP fusion, ensuring receptor functionality and collagen interaction without impacting fluorescence.


Source

DDR domain derived from mammalian sources, with EGFP from BBa_K1911005.

References